Survey of HPV genotypes using multiplex PCR and flow through hybridization in Jakarta, Indonesia

Abstract

This is a study to identify genotypes of Human Papilloma Virus (HPV) among women in Jakarta. The virus increases the risk of cervical cancer, which the second highest incident of cancer in Indonesia. Although there is vaccination program to prevent infection of HPV, it must be based on prior screening of recipients based on HPV genotypes. Each genotype has different potential causing premalignant lesion. Most previous studies are not defining the genotypes of HPV. Therefore, this study is conducted to survey genotypes of HPV using Multiplex PCR and Flow Through Hybridization. The Multiplex PCR and Flow Through Hybridization can detect 35 genotypes, consisting of 18 high risk and 17 low risk genotypes. Samples are patients who visit a clinic in Jakarta. Most of them are women who are already married and between 20 and above 50 years of age. Samples are collected between August to November 2022. There are 336 samples. The result shows that 53.57% of samples were detected positive for HPV. The most prevalent high-risk genotype is genotype 52 while the most prevalent low risk is genotype 11. There are 15,74% of cases of co-infection. The genotype 11 is the genotype that are the most often found in co-infection. The finding is not reported in previous studies. The Multiplex PCR and Flow Through Hybridization can detect genotypes of HPV.