Detection of Sexually Transmitted Diseases Using the STD Direct Flow Chip in Jakarta Special Region, Indonesia

Abstract

Sexually Transmitted Diseases (STDs) remain a major global health concern, with millions of new cases annually. Traditional diagnostic methods, which require separate testing for different pathogens, are time-consuming and costly, hindering disease control efforts. The increasing prevalence of co-infections further emphasizes the need for simultaneous pathogen detection. This study evaluates the effectiveness of the HybriSpot 12 Manual platform and the STD Direct Flow Chip kit in detecting multiple STD pathogens in DKI Jakarta and analyzes infection distribution patterns by gender. Multiplex PCR and hybridization techniques successfully detected 11 pathogens simultaneously, including 8 bacteria, 2 viruses, and 1 protozoon. Among 432 patient samples tested at IMOQ LAB, 88 men and 73 women had single infections, 46 men and 48 women had co-infections, while 120 men and 53 women tested negative. These findings highlight the necessity of efficient diagnostic techniques to enhance STD surveillance and control efforts.