TESTING OF TOTAL PHENOL AND TOTAL CHLOROPHYLL ETHANOL EXTRACT OF POTATO LEAVES (Solanum tuberosum L.) AFTER APPLICATION OF Trichoderma sp.

Abstract

Potato plants are one of the main commodities in Indonesia which have high nutritional content and are beneficial for the body, one component of which is phenol compounds. Trichoderma sp. application on potato (Solanum tuberosum L.) is used as a biofungicide that can reduce fungal infections. The purpose of this study was to determine the levels of total phenol and total chlorophyll in the leaves of potato plants that had been given Trichoderma sp. The design of this study used a completely randomized design (CRD) with each study being repeated three times. The method used to determine the total phenol content was the Folin-Ciocalteau method. The chlorophyll content in potato leaves was tested using a spectrophotometer with wavelengths 663 nm and 645 nm. The results showed that the effect of the application of Trichoderma sp. has different effects. The average total phenol content of the control sample, the 60 days old sample, the 65 days old sample, and 70 days old sample in a row were 310.31 µg GAE / ml (0.310% w/w), 341.26 µg GAE / ml (0.341% w/w), 213.96 µg GAE / ml (0.213 % w/w), and 325.79 µg GAE/ml (0.325% w/w). The total chlorophyll in potato leaves without treatment was N0 32.01 mg/L and N1 31.36 mg/L. The total chlorophyll of potato leaves treated with Trichoderma was P1 34.37 mg/L; P2 52.9 mg/L; and P3 57.14 mg/L. Trichoderma treatment was proven to increase the chlorophyll content in potato leaves.